Re: [DIYbio] Re: What's wrong with open source PCRs

On 01/28/2016 07:33 AM, BraveScience wrote:
> Why don't we leave behind our backs those tubes? Come up with something new.
> Kind of muhammad story for molecular biology, if you cannot bring the heat to the sample, bring the sample to the heat.
> Samples could move across temperature gradients. You could get either low volume sizes and user friendly equipment.
> Illumina and nanopore are already doing this. All sample preparations is ran on digital microfluidics biochips, although a patent
> fence as high as they sky is out there, and they perform an enriching amplification step previous to sequencing.
>

OK. Idea generating with sample not moving fluidly:
instead of vials,
use well plates with adhesive tape covers and flowing air for the heat flow medium

use pipette tips loaded, then dipped in oil to keep sample from evaporating and flowing air for the heat flow medium.
The thermocycler would look like something with wood pecker hole in it that you put the pipettor and tips into,
then press a button. (Would increase sales of pipettors that have a row of 6 or ten pipette tips -- do they work accurately?)



Idea generating with sample moving fluidly:

Hmm... that idea I just got was too good to blab about right now. This email list of thousands that don't speak at all is a
perfect way to just give away a nice IP kind of idea... Anyone wanting to go to Cork with me re: thermocycling droplet automation?

On 01/28/2016 10:13 AM, Simon Quellen Field wrote:
> Is the expense of the consumables the main reason for going microfluidic? If larger amounts are easier to work with, and the
> consumables were cheap, would people bother with tiny volumes?

I like your lateral thinking:-)

There IS always a reason to be able to process small amounts -- getting an inspiration from an accident where you only have a
little drop of it.

And ease of using hand held pipettors gets left behind when using automation -- then the repeatability and accuracy of machines
has no trouble with 2 uliters

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