Re: [DIYbio] TAE Buffer and pH

> I am mixing up TAE in 50-1 strength with Glacial
> Acetic acid and EDTA added in correct proportions

I hope you're adding tris-base too. Without the tris-base the pH
would be very low due to the acetic acid and would require a lot of
NaOH to bring the pH above 8. If you've added the correct components
in the correct proportions you shouldn't have to adjust the pH, even
if you start with very acidic water.

-cory

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