Re: [DIYbio] What's wrong with open source PCRs

My favorite design (I think I saw it on this list) was the long plastic tube wrapped around a board, with one end of the board in hot water and the other in cold water. The fluid was pumped through the tube, and it alternated being in hot or cold water just by traveling around and around through the tube.

Hard to do with 0.2 ml samples, but it might make a fun science fair project.

-----

Get a free science project every week! "http://scitoys.com/newsletter.html"

On Wed, Jan 27, 2016 at 10:18 AM, John Griessen <john@industromatic.com> wrote:

On 01/26/2016 09:09 PM, Jonathan Cline wrote:

Historically there are thermocyclers designs which use fans and electronically controlled vents to direct heated (or room
temperature) air for assisted ramping.

I think flowing air is the way to lower costs even though it has moving parts which will require engineering.  The moving parts
could be organic piezo material for doors, teeny fan motors with long shafts, and be low cost possibly.  Losing all metal blocks will win out by air stirring.

Here's an example typical use case (synbio purification-ligation) to contrast to your example.


100 cycles      12 °C   60 s

        22 °C   60 s

        12 °C   60 s

        22 °C   60 s
Hold    16 °C   infinite

Is this below office/lab ambient, (requiring refrigeration), a frequent desirable use case?


On 01/27/2016 11:06 AM, Sebastian S Cocioba wrote:
> Think something like this will work? Hand wrapped springs soldered to copper heatsink and thermal-paste-bound to two Peltier.

Yes it will work, but not win the efficiency prize, or the speed of heat transfer prize either with the uneven coil surface.
Stirring the air between the peltiers would be better.  The peltiers's masses will slow the ramp when you reverse and go from cool to heat pumping.

If you could find a tiny motor/propeller from a toy battery powered boat to put in between there it would help.  Trim the plastic propeller to fit, and lose the coils of wire...

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
--- You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/56A90A04.1060801%40industromatic.com.

For more options, visit https://groups.google.com/d/optout.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAA0yOM45h3e0JgHaqPCj9JnGfbBJMaYjHAhtJfwqHkzbYwGgWA%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.