You might avoid all the hasle but it will cost alooot. What i can sujest is. Optimize bacterial chromosome to rat and then create an artificial chromosome and integrate it into rat stem cells which will then can be converted to other tissues or adipocytes at least.
There are very many promoters and ori sites that has to be replaced so the synthesis of such project will be. Probably in 10s of thousends just for the dna itself.
I would say it is doable but super expansive and probably will require something like craig venter institute to complete
On Jan 28, 2016 3:11 PM, "Finn Daffron" <finndaffron@gmail.com> wrote:
-- I am trying to create a batch of thermococcus gammatolerans. This is because I want to use CRISPR to inject thermococcus gammatolerans into rats in order to create radiation resistance with possible implications being people working in nuclear power plants, astronauts, etc. Does anyone have any ideas on how it would be synthesized? Please get back to me.--
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