Re: [DIYbio] Re: Anyone have a method of synthesizing thermococcus gammatolerans?

The paper doesn't mention backups for chromosomes to template off of for repair. It does mention major rearrangements in genome of either the species or the genus reconstitution. It is still dependent on the same divalent cations and chromosome condensing proteins to keep everything in place after breaks. This limits how far two strands that were together migrate away from one another. This puts a limit for an otherwise illegitimate end joining.

It could be that the species or the strain or the like has had their evolutionary training in a natural nuclear fission reactor (as in not man made). Yeah, those environments do exist. In fact nuclear material mining companies are surprised by the low yield sources when they hit unusually high output radiation source. Perhaps it was so close to radiation, it was forced to come up with new survival strategies.

Getting back to the Cas9 topic: I read somewhere that it hangs around hours after making the cut. If it does stay on for that long, it won't give you ligation or repair for at least as long. Define quick again? In any case, it definitely doesn't have type II CRISPR. Do to lack of interest in other CRISPR systems little is known about their properties.

Water is that species' natural environment, so cells in water quench ROS much quicker than those w/o water. That's another factor.

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