Hello Finn,
Havent really followed the conversation this far, so apologies if I'm repeating somebody else. ... What is your specific hypothesis? You can't just throw an entire bacterial genome into a eukaryote cell. I think radiation resistance is about very efficient / active DNA damage repair? So which pieces do you wish to edit?
>matt
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On Feb 3, 2016 9:15 AM, Finn Daffron <finndaffron@gmail.com> wrote:
Thank you for all of your answers. My idea was to use UV lights as radiation. I am glad that people believe that that is a good idea. What do you think of using PCR to insert thermococcus gammatolerans DNA into the sperm of rats as opposed to CRISPR?
On Thursday, January 28, 2016 at 3:11:22 PM UTC-5, Finn Daffron wrote:
-- On Thursday, January 28, 2016 at 3:11:22 PM UTC-5, Finn Daffron wrote:
I am trying to create a batch of thermococcus gammatolerans. This is because I want to use CRISPR to inject thermococcus gammatolerans into rats in order to create radiation resistance with possible implications being people working in nuclear power plants, astronauts, etc. Does anyone have any ideas on how it would be synthesized? Please get back to me.
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