Perhaps I'm not caught up on the thread but shouldn't your primers be in micromolar concentrations? At the least a few hundred pico
On Mar 1, 2016 9:17 PM, "David Ishee" <midgardkennels@gmail.com> wrote:
-- The second reverse primer I tried was
CTCGAG
TTAATCCTTGATATTCTTTTG
That was a reverse primer made for LuxE
I made the same mistake with the restriction site added to the end.
--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/8bed0d89-3d6b-4491-8051-397c7c885eb8%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAGdeWmRromxM4xe1bQA0aQfa6ERztxv19Qg5cQx-Ggkfaqq8AQ%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment