[DIYbio] Re: Beginner questions!

All the other places have the same rules except perhaps the-odin, though I don't know if they have a good beginner GFP kit. You'll have to get it sent to a school or business. 

-Koeng

On Friday, April 1, 2016 at 3:37:57 PM UTC, Towa wrote:
Hi guys, I'm in a similar place as Justin. Several of you recommended the GFP kit from Carolina.com, but it seems like they only ship these kits to schools and businesses. Is there another place where I could get a kit?
thanks!

On Wednesday, March 23, 2016 at 1:02:21 AM UTC-4, Justin S wrote:
Hi all,

I'm a total beginner, but I want to get started doing some experimental biology of my own. I'm hoping the community here can help fill in some gaps for me!

1) Suppose I want to modify the DNA of some bacteria with the goal of producing some protein. How does one go about figuring out what sequence needs to be added to the bacterial genome?
2) How is the sequence inserted? I'm aware of crispr, but I'm guessing there are other techniques. What's available to the layperson?
3) How does one verify the desired proteins are produced? 


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