All,
-- I am very interested in this topic. To transform filamentous fungi it is most practical to use electroporation. There are very few protocols that are established for chemical transformation. If anyone has ideas for how to get this to work well please post or share the ideas. The amount of electrical engineering involved in this is beyond my skill level.
-Will
On Friday, April 29, 2016 at 10:21:39 AM UTC-4, Kermit Henson wrote:
On Friday, April 29, 2016 at 10:21:39 AM UTC-4, Kermit Henson wrote:
Hi,Maybe you already know about this paper, but here it goesits a diy electroporator for 50usd.the problem i see is that they use glass capillaries (yes, you can build a glass puller too T_T), but i think it may work for bacteria with some small modificationsEl jueves, 28 de abril de 2016, 22:14:16 (UTC+2), Nathan McCorkle escribió:> It'd also be simple enough to add measurement of the discharge curve using a
> voltage divider and the built in ADC of an atmega chip.
>
> Add an ESP8266 and you'd be able to connect via wifi+web to download and
> view the conductance measurement and discharge graph for use in your
> scientific article.
ESP8266 has an ADC, so no need for an arduino/atmega, most likely.
--
-Nathan
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