[DIYbio] More beginner questions

Starting to develop a keen interest in this topic.  First post.  

Let's say I have a viable bacterium (with my protein of interest inserted into a plasmid) and i inoculate a suitable growth media with my e. coli.  

1. Do I need to anything special to ensure that my protein of interest gets expressed?  
2. After the growth phase has run its course, how to i extract my protein of interest?  (I assume there's not just one way, just interested in approaches to purification)

Thanks!

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