Starting to develop a keen interest in this topic. First post.
-- Let's say I have a viable bacterium (with my protein of interest inserted into a plasmid) and i inoculate a suitable growth media with my e. coli.
1. Do I need to anything special to ensure that my protein of interest gets expressed?
2. After the growth phase has run its course, how to i extract my protein of interest? (I assume there's not just one way, just interested in approaches to purification)
Thanks!
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