Hey Bryan,
First I would say don't spend $60 on that kit. We have much better prices http://the-odin.com. Any sterile swab will do, you can find some pretty inexpensively on Amazon.
You can probably get away with storing the cells in water. 50mM NaOH 50mM TE is probably best but you can probably get away with 50mM NaOH or 50mM Tris or TE or most any buffer. The spontaneous hydrolysis half-life of DNA is something like 50,000 years so you are pretty much only worried about DNases, even so, the chances that they chop up your gene of interest(PTC) in all the genomes is small.
Feel free to email me with any questions if you need more help.
Josiah
On Wednesday, June 22, 2016 at 4:55:51 AM UTC-7, Bryan Daniels wrote:
-- First I would say don't spend $60 on that kit. We have much better prices http://the-odin.com. Any sterile swab will do, you can find some pretty inexpensively on Amazon.
You can probably get away with storing the cells in water. 50mM NaOH 50mM TE is probably best but you can probably get away with 50mM NaOH or 50mM Tris or TE or most any buffer. The spontaneous hydrolysis half-life of DNA is something like 50,000 years so you are pretty much only worried about DNases, even so, the chances that they chop up your gene of interest(PTC) in all the genomes is small.
Feel free to email me with any questions if you need more help.
Josiah
On Wednesday, June 22, 2016 at 4:55:51 AM UTC-7, Bryan Daniels wrote:
Nathan and Kermit - Thanks very much for your response and help.
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