I was trying something along the paper attached bellow but it was very finicky since I didn't have correct buffer and my pvp is lower MW. It should work as they showed in that paper but I need more time to nail it down (also order correct buffers). Since I am going away for next 2 months, I just wanted to share what I had till now just in case someone else wants to do their own stuff. The whole capacitive sensing is very powerful, and simple enough in my opinion so I just wanted to share.
On Friday, June 17, 2016 at 4:35:10 PM UTC-5, Nathan McCorkle wrote:
-- On Friday, June 17, 2016 at 4:35:10 PM UTC-5, Nathan McCorkle wrote:
Nice! So it seems you never tried it with DNA? From what I can
remember, mass-to-charge is constant regardless of DNA length, thus
you need activated capillary walls to aid effect separation.
(easiest to read reference on internal coatings for DNA
sequencing/separations I could quickly find is pg 130 here:
https://www3.appliedbiosystems.com/cms/ )groups/mcb_support/documents/ generaldocuments/cms_041003.
On Fri, Jun 17, 2016 at 11:24 AM, Gordana Ostojic
<gordana....@gmail.com> wrote:
> Here is a capillary electrophoresis setup that I made
> http://www.instructables.com/id/Capillary-Electrophoresis/ . Ultimately I
> think it can be used as a replacement for gel electrophoresis for bio DIY
> but I am not going to able to work on it next 2 months so I kinda wanted to
> just update you people, in case someone wants to take their own take on it.
> Also I think some parts can be used for standard gel electrophoresis (using
> syringe needles instead of platinum electrodes could possibly work even
> though higher current in GE could be the culprit).
>
>
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