Re: [DIYbio] Capillary electrophoresis that I made but can be improved

On 06/20/2016 11:59 AM, Gordana Ostojic wrote:
> I initially started with this HV - 2KV
> <http://www.ebay.com/itm/High-Voltage-Power-Supply-Arduino-HV-Adjustable-DC-2200V-Over-2kV-/252367544293?hash=item3ac2471fe5:g:2UkAAOSwjVVVvRse> and
> that one discharges slowly. I got myself shocked couple of times before I made a parallel connection with a switch and a light
> bulb to discharge it. I thought that one can put in-line fuse with some limiting current to prevent large current going through
> you or equipment, or in my case I used batteries to supply input voltage (that limits how much energy it can discharge so I felt
> safer). Also, you can discharge it by touching electrodes but it does produces awful spark and it is not good in the long term for
> electrodes or circuits around.

In reverse engineering laser printer HV supplies for the electroporator project, I have seen they use commodity
HV resistors of extremely high values as bleeder resistors so the volts goes down rather quickly and also allows swift charge up.
They are through hole parts with a long slab of silica or alumina and two terminals with values such as 200megohm, 400 megohm
1.2 gigohms. Those values are higher than a bare board after you touch it with a sweaty finger and it dries out...
Just for bleeding off charge for safety purposes. In the photo, they are the blue flat rectangles and the yellow larger flat
rectangle.

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