70% ethanol is commonly used to preserve tissue samples prior to gDNA purification/extraction and then PCR. As I understand it's used a lot in the field for preserving samples for DNA barcoding. It's simple, affordable, and effective.
Unfortunately, usps regulations are extra restrictive when it comes to shipping ethanol in particular, as opposed to isopropyl alcohol, methanol, TE, other complex buffers etc. Probably intended to regulate access to intoxicating beverages via the mail, but I don't believe they include exceptions for research samples. Do some googling if you are concerned about that, or check ups / fedex / dhl regs.
On Wed, Jun 22, 2016 at 8:45 AM Josiah Zayner <josiah.zayner@gmail.com> wrote:
Hey Bryan,
First I would say don't spend $60 on that kit. We have much better prices http://the-odin.com. Any sterile swab will do, you can find some pretty inexpensively on Amazon.
You can probably get away with storing the cells in water. 50mM NaOH 50mM TE is probably best but you can probably get away with 50mM NaOH or 50mM Tris or TE or most any buffer. The spontaneous hydrolysis half-life of DNA is something like 50,000 years so you are pretty much only worried about DNases, even so, the chances that they chop up your gene of interest(PTC) in all the genomes is small.
Feel free to email me with any questions if you need more help.
Josiah--
On Wednesday, June 22, 2016 at 4:55:51 AM UTC-7, Bryan Daniels wrote:Nathan and Kermit - Thanks very much for your response and help.
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