Re: [DIYbio] Re: Wild-type organisms suitable for plasmid extraction?

Do you need a lab for this? Some countries just won't care as long as it's not GMO. You can use a pressure cooker, incubate it on a bookshelf and buy a miniprep kit and do gelelectrophoresis in the garage. Avoid Ethidium bromide though.




On Friday, July 22, 2016 at 12:31:00 AM UTC+2, jarlemag wrote:

Thanks for the offer - maybe later? We don't have a lab space for microbiology/culturing set up yet.

Best regards,

JP

torsdag 21. juli 2016 22.03.14 UTC+2 skrev Koeng følgende:

I can send you a sample if you want.

-Koeng

On Thu, Jul 21, 2016 at 7:46 PM, jarlemag <jarle...@gmail.com> wrote:

Thanks for the tip, that's interesting. From what I'm reading after some googling it seems that Mutaflor is not available for sale in Norway though, and might not be legal to import privately due to being considered a pharmaceutical. Maybe it's possible to get the pure strain somehow.

Best regards,

JP

torsdag 21. juli 2016 21.08.34 UTC+2 skrev Koeng følgende:

Apparently E coli nissle has 2 plasmids

http://www.microbecolhealthdis.net/index.php/mehd/article/viewFile/7512/8855

In Europe you can get E coli Nissle 1917 from the probiotic "Mutaflor". You could use basic plasmid miniprepping reagents that companies are always trying to sell. I don't know about copy number, though.

-Koeng

On Thursday, July 21, 2016 at 4:17:00 PM UTC, jarlemag wrote:

Hello,

As part of setting up a DIY bio lab and introducing newcomers to molecular biology, it would be nice to demonstrate plasmid extraction and subsequenct study of the plasmid by restriction digestion or sequencing. The catch is, local regulations prevent us from working with genetically modified organisms, including organisms transformed with engineered plasmids. Are there any wild-type BSL1/generally recognized as safe organisms harboring natural plasmids which might be suitable? A strain that is readily available from a culture collection or from fellow DIY biologists would be preferable.

Best regards,

Jarle Pahr 

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