Re: [DIYbio] Request for Artificial gene synthesis

I have been working on trying to make a complete easy front-to-back
system for microfluidic product, with the intent on DNA synthesis.
Recently I bought this commercial blu-ray laser (XBox of some sort
replacement) that these folks reverse-engineered all the controls for,
and then built a circuit-board to enable all functionality and control
for anyone:
http://www.diyouware.com/DiyoPCB-MKI

This will achieve sub-micron spot and feature sizes, enabling
microfluidic circuitry to be written in photoresist. I have, for now,
purchased a used spin-coater, but I did previously find a vacuum
bearing for about $11 which would enable a spin-coater to be built
with a BLDC motor and simple vacuum pump pretty easily. I have been
focusing more on the CAD and CAM things lately (I have local changes
that I have been meaning to push, such as a 3-finger peristaltic pump
model):
https://github.com/nmz787/python-brlcad-tcl/tree/master/python-brlcad-tcl

some kicad models (electrical not fluidic) converted to g-code,
direct-laser exposed photoresist which I spincoated:
http://imgur.com/a/mQbYA
and after inverting the exposure and trying again:
http://imgur.com/aCaCTJJ

there is also chatter here once in a while:
https://groups.google.com/forum/#!forum/enzymaticsynthesis

On Wed, Jun 29, 2016 at 10:29 AM, Shambo Hore <shambo.hore88@gmail.com> wrote:
> Dear all.
> Please refer any DIY method for artificial genetic code synthesis. If there
> any ongoing project regarding with this matter, i will be glad to
> participate with it.
>
> Thank you.
> Shonku.
>
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--
-Nathan

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