Re: [DIYbio] Using isopropanol in place of ethanol in DNA purification kits?

On Sat, Jul 23, 2016 at 1:46 AM, jarlemag <jarle.pahr@gmail.com> wrote:
> Thanks for the advice, everyone. I'm familiar with the differences in pellet
> visibility, but I didn't know about the difference in salt solubility. In
> any case, I'm not concerned with manual pelletting and washing, but spin
> column kits like this one: http://dongshengbio.com/en/xxcp.asp?id=271

As described in that protocol "Discard the flow-through", the reason
for the alcohol is to remove salts or other impurities. Because
ion-affinity chromatography is based on charge, this is largely based
on pH, but salts also affect this and sometimes the buffers include
salts specifically to enhance binding for example. So use less
isopropanol in place of the ethanol.

P.S. Cory confirmed he has tried this with spin-columns and it works

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