A couple main things-
Developing open source plasmid vectors and easy cloning methods. This has taken a massive amount of time, far longer than I expected, because I've been a perfectionist with base plasmid design, although I've gotten some great data lately on my cloning methods. Right now I'm in the process of finishing a few of the base plasmids, characterizing phage packaging, and Vibrio natriegens transformation.Protein-primed replication in bacteria
I work in a lab based on this technology and I want it working in bacteria. It is based on pBClin15 and hypothetically I may be able to get an orthogonally replicating plasmid (though I have doubts on true orthogonality)
-Koeng
On Wednesday, August 17, 2016 at 11:54:21 AM UTC, Michael Flynn wrote:
On Wednesday, August 17, 2016 at 11:54:21 AM UTC, Michael Flynn wrote:
I read a lot of the topics on this board, a lot of questions of how to do this, how to fix that. I was wondering what everyone was trying to do with these cool DIYBio projects. So what are you working on?
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