Re: [DIYbio] What are you working on?

CRISPR knockout of the arabidopisis flowering gene. I am at the ligation step.

CRISPR knockout of the arabidopisis flowering gene. I am at the ligation step.

Dan

On Wed, Aug 17, 2016 at 2:00 PM, Nathan McCorkle <nmz...@gmail.com> wrote:

Microfluidic production using open-source software and tools. Lately
not much progress, but I was last working on a way to easily describe
the connectivity of lab-on-a-chip components easily through Python
code, which in turn talks to BRL-CAD to generate physical models,
which can then be exported to STL files (think 3d printer software) or
g-code (think CNC machines, laser etcher/exposers) or Bitmap files
(think lithography masks, stencils, images for projection using DLP
projector).

Specifically the last thing I was working on was an API for connecting
CAD 'objects' using fluidic ports, such as the center points of each
of the 5 connections on this peristaltic pump:
https://github.com/nmz787/python-brlcad-tcl/blob/master/examples/output/microfluidic_pump.stl

(3 pneumatic connections, 2 fluidic) (notice the pneumatic connections
can come from either side, so maybe there are actually 6 options for
pneumatic connection points)

I've also been investigating how to command the NIRT tool, to take the
BRL-CAD model and generate g-code from the layers/slices of the model.
Then I can take that g-code and throw it into my laser etcher,
exposing some photoresist,  repeat with new photoresist slide for each
layer, then combine layers (back to back) (or, pour silicone on, later
peel it off, then make a sandwich between glass plates).

On Wed, Aug 17, 2016 at 4:54 AM, Michael Flynn <mfly...@gmail.com> wrote:
> I read a lot of the topics on this board, a lot of questions of how to do
> this, how to fix that. I was wondering what everyone was trying to do with
> these cool DIYBio projects. So what are you working on?
>
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-Nathan

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