Just learned about the plethora of information starting from Gibson assembly and came across this post.
If this is not you, someone have already tried the PEG8000 approach and pervailed :-) http://openwetware.org/wiki/SPLiCE
What I wonder is however this protocol also do not mention about inducing the pKD46 plasmid, is it expected (kind of D'uh! moment?) or it just leaks enough to make a difference (since it is araB, one should not expect leakage).
-- If this is not you, someone have already tried the PEG8000 approach and pervailed :-) http://openwetware.org/wiki/SPLiCE
What I wonder is however this protocol also do not mention about inducing the pKD46 plasmid, is it expected (kind of D'uh! moment?) or it just leaks enough to make a difference (since it is araB, one should not expect leakage).
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