In my most recent test, without pKD46, I got 10 colonies from the SLiCE reaction, which was the same as the no SLiCE control. SLiCE with pKD46 resulted in 50-60 colonies. For comparison, NEBuilder resulted in about 100.
On Thu, Oct 20, 2016 at 8:52 PM Nathan McCorkle <nmz787@gmail.com> wrote:
On Oct 19, 2016 11:21 AM, "John Griessen" <john@industromatic.com> wrote:
>> Isn't the number after the PEG acronym for length or molecular weight? Its property of interest is absorbing/adsorbing water,
I've heard it can also be used for 'molecular crowding'. The longer the molecule, the thicker the molecular crowd in that area of volume, because the more would-be monomer can't diffuse from the rest of the polymer.
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