Hello, I'm a high school student and I'm working on a school project to produce spider silk in vitro. Part of this project is to find the best media/ringer solution to keep the tissue alive the longest. The goal is to produce the silk at as low as a cost as possible, so I was thinking of cheap ways to get the nutrients it needs. Since it's an organ culture, it won't last much longer than a couple of hours, but I need to supply it with amino acids so it can keep producing silk.
-- So, I was thinking of using an UV light to kill the yeast then incubating it (at 45C?) for a time period. Is this a dumb idea? Will the UV light be effective in killing the yeast and not causing an infection? How do I make the extract suitable for tissue culture?
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