[DIYbio] How to create a yeast extract from bread making yeast for medium?

I've made custom yeast extract for some Mesoplamsa cultures. The instructions on how to make the media were so old that it referenced how to make your own media.

Don't do it yourself. It is a bad idea. I spent more money on purification stuff (filters and such) to get enough of it sterile than I would have paid if I got it through a company. And, may I note, this was 15% yeast extract which is 80$ for 500ml (I followed the protocol but dang). Just buy it. I'm sure there is some long lost method on how to efficiently do it out there in old literature, but if you are a high school student, just buy it.

If anyone has a better way I'm all ears, but my experience has been rather bad with diy media components

-Koeng

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