[DIYbio] Re: How to create a yeast extract from bread making yeast for medium?

You can buy baker's yeast and do the extract directly from that or growing the yeast. If the later, you need to separate the cells from the culture. You shouldn't lose too much yeast filtering with some coffee filters. Afterwards, you have 2 options:
- resuspend and autoclave directly: you may want to let it settle before you use it either if you want to filter it or not to avoid all the solid cell walls before mixing with the other sterile media components.
- resuspend, cook at 45-55 °C, filter and dry the extract. Then add as you require to the medium and autoclave. You may do this and keep the suspension in the fridge for a short time but I wouldn't use it after 48h even if it was kept in the fridge



On Wednesday, 2 November 2016 15:01:53 UTC+11, Aubrey wrote:

Hello, I'm a high school student and I'm working on a school project to produce spider silk in vitro. Part of this project is to find the best media/ringer solution to keep the tissue alive the longest. The goal is to produce the silk at as low as a cost as possible, so I was thinking of cheap ways to get the nutrients it needs.  Since it's an organ culture, it won't last much longer than a couple of hours, but I need to supply it with amino acids so it can keep producing silk.  

So, I was thinking of using an UV light to kill the yeast then incubating it (at 45C?) for a time period. Is this a dumb idea? Will the UV light be effective in killing the yeast and not causing an infection? How do I make the extract suitable for tissue culture?