I have been working on the APHIS level 2 account, but you need to pay $150 for the VS16-3 form to get the bacteria, but if you request it from within the same state, I don't need permission right? I have never done this before, but could I just email a University and ask them to send me some bacteria if they are within the same state?
On Sunday, November 6, 2016 at 7:08:40 AM UTC-6, Sebastian wrote:
-- On Sunday, November 6, 2016 at 7:08:40 AM UTC-6, Sebastian wrote:
GV3101::pMP90RK is what I meant. Thanks for bringing that up! Its genomically resistant to rifampicin and the Ti plasmid has gentamicin resistance. Its a solid strain!
Sebastian S. CociobaCEO & FounderNew York Botanics, LLCBlog: ATinyGreenCell.comGood day,
when you say GV3101, are you talking about the cured strain without its helper plasmid? Or do you mean GV3101:pMP90 or pMP90RK? It is my understanding that without its helper plasmid the strain is not virulent and can therefore not transfer any DNA to the host plant.
Cheers,
Johannes
Am 1/11/2016 um 2:55 AM schrieb Sebastian S Cocioba:
My personal favorite is GV3101 from Stan Gelvin's Lab at Purdue. EHA and LA are both decent but I like the aggression of the GV line.
Sebastian S. CociobaCEO & FounderNew York Botanics, LLCBlog: ATinyGreenCell.com
--Is it hard to get approved? Do I need the form each time? What strain should I get?--
On Monday, October 31, 2016 at 4:35:13 AM UTC-5, cory....@gmail.com wrote:-coryFor domestic transportation of Agrobacteria you need permission from USDA APHIS which you can request using form PPQ-526. Plan a month or so in advance because they're kind of slow.If you plan on transporting plants that were genetically transformed with Agro you'll need USDA APHIS form 2000. Although, Arabidopsis thaliana is exempt.
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