Re: [DIYbio] AgroBacteria DIY BIO DALLAS

No worries.

I ask because I just read a paper where the authors say they compared 3 different strains of Agro, amongst them GV3101 and mentioned that efficiency with that strain was low. But then in their supplemental data they mentioned they used the cured strain. So after contacting the author and asking him about why they used the cured strain, which would be avirulent and SHOULD have low efficiency, if any at all, they said they said it's because many labs use it, and they were not sure which strain exactly it was (with or without Ti plasmid) and that that information may have been lost over the course of the experiments....

GV3101::pMP90RK is what I meant. Thanks for bringing that up! Its genomically resistant to rifampicin and the Ti plasmid has gentamicin resistance. Its a solid strain!

Sebastian S. Cocioba

CEO & Founder

New York Botanics, LLC

Blog: ATinyGreenCell.com

On Nov 6, 2016, at 6:05 AM, Johannes Debler <johannes.debler@gmail.com> wrote:

Good day,

when you say GV3101, are you talking about the cured strain without its helper plasmid? Or do you mean GV3101:pMP90 or pMP90RK? It is my understanding that without its helper plasmid the strain is not virulent and can therefore not transfer any DNA to the host plant.

Cheers,

Johannes

Am 1/11/2016 um 2:55 AM schrieb Sebastian S Cocioba:

My personal favorite is GV3101 from Stan Gelvin's Lab at Purdue. EHA and LA are both decent but I like the aggression of the GV line. 

Sebastian S. Cocioba

CEO & Founder

New York Botanics, LLC

Blog: ATinyGreenCell.com

On Oct 31, 2016, at 6:16 AM, Josh Melnick <j.r.melnick@gmail.com> wrote:

Is it hard to get approved? Do I need the form each time? What strain should I get?

On Monday, October 31, 2016 at 4:35:13 AM UTC-5, cory....@gmail.com wrote:

For domestic transportation of Agrobacteria you need permission from USDA APHIS which you can request using form PPQ-526.  Plan a month or so in advance because they're kind of slow.

If you plan on transporting plants that were genetically transformed with Agro you'll need USDA APHIS form 2000.  Although, Arabidopsis thaliana is exempt.

-cory

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