Welcome to the wonderful world of plant tissue culture!!!! ::confetti::
Orchids bleed and their blood is toxic to them. Use activated charcoal in orchid media.
Don't start with random plants. Start with tobacco seeds and surface sterilize them. Sprout them and use the resulting aseptic seeds as leaf tissue stock for subsequent practice cultures.
10% household bleach, drop of dish soap, and pre-wash the plant tissue with soap and water manually to get macro-particles off. Soak in bleach for 10-30mins depending on surface area and convoluted tissue structures. Rinse 3x in sterile distilled water. Buy a heap of 6" tweezers and use one tweezer per culture vessel to not spread contam culture to culture.
Orchid media works best with coconut water, a banana, and sucrose. Phytotechlab.com sells everything you need. Plants From Test Tubes 4th edition was my bible when I started. Keep the thread alive and I'll chime in. Best of luck!!!
Sebastian S. Cocioba
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Blog: ATinyGreenCell.com
We are working on building a space here in Dallas, so far our first attempt at Tissue Culture has been rather poor.--About 65% of the vials have mold or fungis, we have quite a good autoclave here, so I will have to work and be much more careful in the future about contamination when putting the plants in the tubes.I put orchid and rose in the tubes, using multi solution from MicroClone. What is interesting is this black coloration that is coming from the orchids, I suppose this is some product of the plant's metabolism as it is not coming into the other rose plants. I probably should start a blog.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/0ea53f4d-edef-4eb0-b898-ccb1b083615f%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
<IMG_0911[1].JPG>
<IMG_0908[1].JPG>
<IMG_0910[1].JPG>
0 comments:
Post a Comment