Seriously, not worth doing a DIY sequencer. I worked at Applied Biosystems, the guys who commercialized the fluorescent gel based sequencer, then the fluorescent capillary sequencer. And at Illumina who commercialized next generation sequencing. It is a lot of work, mostly in optimizing.
Have attached a graphic which summarizes most of the sequencers out there.
For the gel based - yes you can DIY but you will need.
a) Long glass plates (at least 30-50 cm long) and spacers to create the parallel vertical plates to pour the gels in
b) a high voltage power supply - about 3000 V that is > 100W
c) acrylamide monomers to create the gel. These are neurotoxic.
d) Labeled sequencing primers (with fluorescent dye or with radioactivity)
e) Pure DNA templates (what you want sequenced) with adapters stuck on the ends (region where the sequencng primer will bind) - can be created by PCR
f) Sequencing mix - contains dideoxy terminated nucleotides with normal nucleotides. Enzymes, buffers, etc.
g) ability to dry the gel and expose to a large X-ray film of using radioacivity.
The bible for capillary sequencing is at this link
On Fri, Nov 4, 2016 at 1:15 AM, Hugues <laliberte1965@gmail.com> wrote:
But wait, i'm confused now. I thought we could read a DNA sequence with gel electrophoresis, no ?--Isn't what we can Sanger sequencing, with the help of dideoxynucleotides ?example here:but i'm new to this, so maybe i missed something ? or this method works but it's too long and nobody uses it anymore ?
On Friday, 4 November 2016 04:07:59 UTC+1, SC wrote:That isn't a sequencer. It's a PCR machine and an electrophoresis unit. You won't be able to get sequence out of it.
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