Re: [DIYbio] Using dietary amino acid supplements in media

On 11/07/2016 09:23 PM, Aubrey wrote:
> ringer solution, but with added amino acids that the gland uses up rapidly such as valine, methionine, threonine etc. In order to
> put only these solutions into the media

Sterilizing with a micron filter seems OK for your uses of a few hours for an insect part. Doing careful quantitative
preparations with Ringer's and also everything-else-the-same-except-one-new-variable, Maramorosch Medium, swapped for Ringer's
would be a good read. Then if all that gives results, you could try flowing new solution past the spinarette
to see if time of silk production could increase. Any of those experiment preparations compared would be a good read.

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