Hi guys,
-- i think i have successfully CRISPRed an E. Coli bacteria,
I have used the kit sold here:
http://www.the-odin.com/diy-bacterial-gene-engineering-crispr-kit/
I followed the protocol here:
https://docs.google.com/document/d/19RNlo-psr_kTeJtKOZK6ruFW18Uu7SIX4zZtHSc9dzQ/edit
The gene that was targeted is Escherichia coli 30S ribosomal subunit protein S12 (rpsL) gene. One base pair was changed to render the bacteria resistant to streptomycin.
The picture attached shows my result. That plate contains a gel with streptomycin. The top part shows growth on the CRISPRed strain, the bottom part shows not growth on the normal strain.
What you guys think ?
I'm thinking of having the DNA of that gene sequenced, just to check if the edit really happened only where expected.
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