Re: [DIYbio] Finished making a biosafety cabinet aka tissue culture hood

Do some sterility tests too. Leave some rich nutrient media plates like SOC or SOB media with no salt in the hood and open for 15,30,1hr,12hrs and incubate for two or three days at room temp and/or 30C. That should indicate if the hood is cleaning enough of the air. Have some plates outside the hood too. Maybe open one and do a pirouette to catch the particulates in the air around you. Good job on the instructables! 

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC


On Dec 16, 2016, at 10:36 AM, Abizar Lakdawalla <abizarl@gmail.com> wrote:

Details for a diy tissue culture sterile hood made out of a room HEPA air purifier are at http://www.instructables.com/id/Biosafety-Cabinet-for-Tissue-Culture/

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