Re: [DIYbio] Re: Where can i get an E. Coli gene sequenced for cheap ?

Two kinds. If you are sequencing a gene of less than 1000 bp then you would use Sanger sequencing which is read by capillary electrophoresis. That costs about $10. If you are sequencing the whole Genome of E.coli, 5,000,000 bp, then that would cost $100's.

On Dec 29, 2016 8:51 AM, "Hugues" <laliberte1965@gmail.com> wrote:

Hello,

10 to 20 usd ??

mmm....

Here is a quote i received , for 760.80 usd for 2 samples, shipping charges excluded

Are we talking about the same work here ? Sorry i'm a newbie to this.

Can you give me the name of a company doing this work for that kind of price ? I will contact them.

***************************

Our Offer

1. DNA isolation

DNA isolation (column) 2 - 160.40 320.80

Total 320.80

- DNA will be isolated from your samples using an appropriate kit. 

2. Primer design & synthesis and PCR testing and optimization

Primer design including synthesis 2- 25.00 50.00

PCR set-up and optimization 1 300.00 300.00

Total 350.00

3. PCR clean-up and Sanger sequencing

Single PCR purification, price per sample  2- 9.00  18.00

Economy Run 4- 18.00  72.00

Total 90.00

- PCR products will be purified and bi-directionally Sanger sequenced. The results of the Sanger

sequencing will be sent to the customer for further analysis.

Total costs for the characterization: CHF 760.80

*****************

On Saturday, 24 December 2016 00:44:52 UTC+1, Mega [Andreas Stuermer] wrote:

Basically any synthesis company will sell you primers ("oligos"). And they will also do a sequencing run. Make sure to inquire about prizing first. Total costs should be 10 to 20$ plus shipment of your DNA to them.



On Tuesday, December 20, 2016 at 6:24:33 PM UTC+1, Hugues wrote:

Hi guys,

I have 2 samples of E. Coli for which i would like to have a specific gene sequenced,

where is the cheapest place i can get that done ?

I checked online, there are many, not sure where to start , any recommendations ?

i live in Switzerland

thanks

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/f7eb41eb-15df-46c4-b3c0-b680b16e6daa%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to a topic in the Google Groups "DIYbio" group.
To unsubscribe from this topic, visit https://groups.google.com/d/topic/diybio/cB-6U66xvgM/unsubscribe.
To unsubscribe from this group and all its topics, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAND8PcObUscEOsaF3eCjPoboArNtwVu_a6%3D5D-4TXaFCQXB%2B3A%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.