For those who want their own drill bits....
El viernes, 16 de diciembre de 2016, 13:53:49 (UTC+1), Pieter Waag escribió:
-- http://www.diybiogroningen.org/mediawiki-1.22.0/index.php?title=OpenerPCR_metal_parts
On the website, you can find this:
"We had our drill bit made by a company called RVP Engineering in The Hague, it's a custom metal manufacturing company. I am sure that there should be companies like that near Groningen. The drill bit costed approx. 80 euro."
It's almost true, but RVP charged us 150e. Im not quite sure if they hire a 3rd party or they do the drills on their own installations :/
I tried to contact with several manufacturers on Texas, but no success. For sure there must be some cheap manufacturer for this job, and it could be a big step on open PCRs machines.
I know that you could use a CNC, but with the this drill bits the job is quite easy and fast.
@Pieter: where do you put the epps? can you show/share some pictures?
El viernes, 16 de diciembre de 2016, 13:53:49 (UTC+1), Pieter Waag escribió:
It would be great if you could share the design files. For those of you that are considering to build one yourself, here are our source files: https://github.com/BioHackAcademy/BHA_ Thermocycler The aluminium block is indeed the only part that was custom made.
On Friday, December 16, 2016 at 12:46:45 PM UTC+1, Kermit Henson wrote:Dont think that pass the drill bits could be an option, sorry. To spend 150e and dont know in which state they will come back, for me (or my group), is not an option. The designs are open, I can send them.What could be an option is to perform the holes in our lab, but the interested should pay all related spends (metal block, transport...) But, like I said, we are still waiting for them
El viernes, 16 de diciembre de 2016, 5:00:51 (UTC+1), Nathan McCorkle escribió:Maybe once your group uses the drill bits, you could pass them along
to another group? Then that group would do the same, and so on.
On Thu, Dec 15, 2016 at 12:38 AM, Kermit Henson <kerm...@gmail.com> wrote:
> MiniPCR is a nice machine, it works as expected but the fan noise can be
> annoying
> Ninja PCR is original from Japan (https://github.com/hisashin/NinjaPCR ), but
> you still hace the problem of the heat block.
>
> The main problem that every DIY-PCR machine faces is the heat-block and how
> to perform the holes for the epps. Anyways, my group already ordered the
> drill bits for the heat block. It costs arround 150-200e. Too much if you
> just want to build one machine, but may be a solution could be using glass
> capilars or something similar.
>
>
>
> El miércoles, 14 de diciembre de 2016, 15:02:37 (UTC+1), Andrew Barney
> escribió:
>>
>> Yeah, i think the OpenPCR project was a success. As far as i know they are
>> still selling them. Should be a good option. The MiniPCR project looks nice
>> also but i don't think that's an Open Source Hardware project. There is a
>> new machine called the NinjaPCR i think designed by students in China, it is
>> based off the original OpenPCR and has some of the same components and is
>> Open Source, but i don't think it is a commercial product (yet?), though it
>> is smaller which looks nice.
>>
>> On Thursday, December 8, 2016 at 11:06:11 AM UTC-7, Bryan Jones wrote:
>>>
>>> I think the OpenPCR project was largely a success. The people behind it
>>> (Chai biosciences) had another successful kickstarter for a qPCR machine
>>> (i.e. PCR w/ optics to measure fluorescence). It looks like their focus is
>>> now pretty much just on the Open qPCR machine and reagents to go with it,
>>> I'm not sure if you can still order the original OpenPCR. Check out @chaibio
>>> on twitter.
>>> The original machine was a good product. I have one that has had regular
>>> use for years and even generated published results. That being said, another
>>> really good option (and probably even more cost effective) is to get a used
>>> thermocycler from ebay, or your local University's used equipment store.
>>>
>>> On Thu, Dec 8, 2016 at 8:44 AM Philip McIntosh <ndpmc...@mac.com> wrote:
>>>>
>>>> We were thinking about getting an OpenPCR machine for our school.
>>>> @openpcr hasn't posted on twitter for quite a while so I am wondering what
>>>> the status of the project is. Anybody know?
>>>>
>>>> Thanks,
>>>>
>>>>
>>>> Philip McIntosh
>>>> Colorado Springs, CO
>>>> ndpmc...@mac.com
>>>> philipmcintosh.com
>>>>
>>>>
>>>>
>>>>
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