Hey Han,
-- I have worked with Yeast a bunch. The SS DNA definitely helps with transformation efficiency but is definitely not required, you will just get less transformants. If you need some Yeast Transformation buffer with SS DNA you can find some here: https://www.the-odin.com/yeast-transformation-buffer/
Thanks,
JZ
On Tuesday, January 10, 2017 at 8:26:55 PM UTC-8, Han Li wrote:
On Tuesday, January 10, 2017 at 8:26:55 PM UTC-8, Han Li wrote:
Hello DIY Bio Community!My name is Han. I am a student who's been interested in DIY Bio, and I wanted to undergo my own project. I have recently been following the JHU 2011 VitaYeast IGEM project (http://2011.igem.org/Team:Johns_Hopkins ) in an attempt to recreate their yeast transformation as a fun way to get started. Electroporation systems have been a little out of my budget for a beginner procedure, so I'm sticking with a lithium acetate transformation instead.However, I have had a bit of trouble searching for salmon sperm DNA that I can use, and I was wondering if you all had any cheap sources for to find it or ideas for replacement materials? I was also wondering how necessary it is to include in the transformation, and if I can simply continue on with just DMSO instead?Thank you in advance, and best of luck in your own projects,Han
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