It gets taken up and does weird things to seedlings. Just use MES buffer at 0.5g/L to handle the variance over time since sucrose metabolism acidifies the media. No need for indicators though it us fun to see the uptake. I played with universal buffers and chlorophenol red.
Sebastian S. Cocioba
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Blog: ATinyGreenCell.com
Hello, after doing some reading on the effects of autoclaving and callus to TC agar media pH, I've come to the conclusion that the actual pH can vary quite wildly with time compared to the measured pH prior to autoclaving. I was thinking if an indicator like phenol red can be added to the media to act as a constant pH indicator. Would this idea work?--
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