The ampicillin resistant bacteria will grow on a media without ampicillin. It will just express the resistance protein but it will not be used for anything.
Over time the bacteria will lose its resistance because of mutations introduced during DNA replication and evolutionary pressure to save energy otherwise spent on producing ampicillin digesting proteins.
On Tue, Apr 4, 2017 at 12:12 PM MC <markyching@gmail.com> wrote:
I have a very basic question as a beginning bio student: What would happen if you sub-cultured something like E. coli with a ampicillin resistant plasmid that's currently growing on amp LB onto plain LB?--My hypothesis based on my limited experience is that assuming no contamination, it will grow but so will E. coli lacking the amp resistance. But why? Do mutations occur in asexual reproduction for the sake of genetic diversity?
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