Re: [DIYbio] Culturing antibiotic resistant bacteria on regular media?

Unfortunately the original reference link is dead, but this says at
incubator temperatures (37 C) ampicillin only lasts 3 days:
http://www.openwetware.org/wiki/Ampicillin#Stability

Just something I came across recently that was interesting to think
about. I sub-cultured some cells and put them in the fridge the next
day for storage, thinking I could get a few weeks of fridge storage
that way, before having to re-culture.

On Tue, Apr 4, 2017 at 9:12 AM, MC <markyching@gmail.com> wrote:
> I have a very basic question as a beginning bio student: What would happen
> if you sub-cultured something like E. coli with a ampicillin resistant
> plasmid that's currently growing on amp LB onto plain LB?
>
> My hypothesis based on my limited experience is that assuming no
> contamination, it will grow but so will E. coli lacking the amp resistance.
> But why? Do mutations occur in asexual reproduction for the sake of genetic
> diversity?
>
> --
> -- You received this message because you are subscribed to the Google Groups
> DIYbio group. To post to this group, send email to diybio@googlegroups.com.
> To unsubscribe from this group, send email to
> diybio+unsubscribe@googlegroups.com. For more options, visit this group at
> https://groups.google.com/d/forum/diybio?hl=en
> Learn more at www.diybio.org
> ---
> You received this message because you are subscribed to the Google Groups
> "DIYbio" group.
> To unsubscribe from this group and stop receiving emails from it, send an
> email to diybio+unsubscribe@googlegroups.com.
> To post to this group, send email to diybio@googlegroups.com.
> Visit this group at https://groups.google.com/group/diybio.
> To view this discussion on the web visit
> https://groups.google.com/d/msgid/diybio/3b0bb6bd-3509-4aab-8e95-7ac02a618b59%40googlegroups.com.
> For more options, visit https://groups.google.com/d/optout.



--
-Nathan

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9Jgr3DJRqST7z0%2B3mueTw5VLsD5uUY%3DkcpWwXHzKWRD4g%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.