Well, it is quite easy to make these substancies known. Just purify them by thin-layer chromatography and make NMR analysis for every spot on the chromatograme. I have access both to the TLC and NMR spectrometer, but have no GelRed or SYBR Safe, but I think here are a lot of people, who have access to these dyes, TLC and NMR. Why not make GelRed and SYBR Safe more known?)
Am Dienstag, 12. März 2013 21:03:42 UTC+2 schrieb Nathan McCorkle:
-- Am Dienstag, 12. März 2013 21:03:42 UTC+2 schrieb Nathan McCorkle:
Yeah, that's why big companies and universities don't switch.
EtBr is molecularly known (no one really knows exactly what the gelRed
and sybrSafe molecules are, we know what classes they're in or one
that was described in one patent, etc)
so it's more 'open' in that sense, that would also probably make it
more reliably modeled in-silico (it's a much simpler molecule as well)
On Tue, Mar 12, 2013 at 11:57 AM, Jeswin <phill...@gmail.com> wrote:
> Hmm, that's what I thought. It seemed like too much. For my 1% EtBr
> gels, its 50mL 1x TAE + 0.5g agarose + 1uL EtBr.
>
> I got enough EtBr to last 100 years at the rate I use.
--
-Nathan
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/42b057eb-9558-42a9-95c6-15ce3924dde9%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment