Re: [DIYbio] Re: SLICE -- Seamless Ligation Cloning Extract... or, using lambda phage homologous recombination in-vitro

Good question, I haven't worked with repeats with slice.

I suspect it'll be heavily dependent on repeat size, number of repeats, content, linkers, where the repeats are in the plasmid relative to place of intended insertion and homology ends, etc. Hard to speculate really...only one way to know for sure. ;)

On Saturday, April 15, 2017 at 3:40:04 AM UTC-7, Mega [Andreas Stuermer] wrote:
What if your plasmid contains repeats? Will there be unwanted recombination?

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