Probably because it requires an extra PCR reaction.
On Wednesday, May 3, 2017 at 11:10:57 PM UTC-7, Michael Crone wrote:
-- The separate SLiCE "enzyme mix" you refer to costs nothing, and with about 2 hrs of prep work one can make enough for 10,000+ reactions. PCRs are not free, often require troubleshooting and can be time consuming. The fewer PCRs one has to do the better in my book.
Granted not all cloning methods work equally well for all situations, but for standard insertion of an insert into a linearized vector it's pretty hard to beat the convenience, cost, efficiency and accuracy of recombinase-expressed cell extract SLICE methods. I suspect those making $$ on Gibson reagents are working hard to ensure these methods don't become commercialized.
On Wednesday, May 3, 2017 at 11:10:57 PM UTC-7, Michael Crone wrote:
Why not just use CPEC instead of requiring a separate enzyme mix just for cloning? Phusion works absolute wonders and I've never had any problem creating large plasmids.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/e4465cb6-2d24-4843-9a22-d447d0e4bf42%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment