Did you grow it at 37C or 30C? 37 deactivates the enzyme
On Sunday, February 26, 2017 at 12:35:16 PM UTC-5, Nathan McCorkle wrote:
-- On Sunday, February 26, 2017 at 12:35:16 PM UTC-5, Nathan McCorkle wrote:
On Feb 25, 2017 9:56 AM, "MC" <marky...@gmail.com> wrote:
>
> That's really interesting, I'm going to have to do some reading on recombination. Both my cultures were DH10B.Oh, ok, I guess the-odin changed how the sell pVIB... their product page showed this week that they ship pVIB in BL21.
Hard to say what could've happened from my end, then.
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