The gene itself is only about 240 bp, so if you can't find it for sale you might be able to build it yourself from long primers.
On Thu, Jul 27, 2017 at 6:17 PM Skyler Gordon <skgor1@gmail.com> wrote:
If you have the capability to buy plasmids, you can most likely buy one that already has GFP cut into it. Other than that I would ask some educational facilities if you can get a sample of the GFP gene to express yourself. If you can't find a plasmid that has GFP and the expression system you want, you may be able to buy the gene from ThermoFisher.
Many companies that sell plasmids won't let you purchase anything under a 'John-Q Public' account so you'll need a business account, non-profit would be the best way to go.
-SGOn Thu, Jul 27, 2017 at 6:01 PM Matt Tucker <mathew.tucker.1@gmail.com> wrote:I found a source for it, and am filing for the USDA permit.
On Thursday, July 27, 2017 at 5:11:56 PM UTC-4, Skyler Gordon wrote:Have you already obtained Agro. spp.? I would look into that before you start trying to get all the other materials.
-SGOn Thu, Jul 27, 2017 at 12:38 PM Matt Tucker <mathew....@gmail.com> wrote:After endless research and reading about genetics, I have finally decided to yield to my curiosity, and put some time into the lab. As such, I'm trying to find an affordable source for GFP to be used in a project where, agrobactarium- t. is used to deliver GFP into a plant. Thoughts?---- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
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