Re: [DIYbio] Gram Staining Problems

On 08/23/2017 10:19 PM, Jonathan Bartlett wrote:
> # Heat fix with butane lighter (3 passes)
> # Apply a few drops of crystal violet and wait 1 minute (NOTE - I don't know if this is unusual, but my crystal violet dries out
> about halfway through the minute).

I have not done this procedure ever, but...

If your crystal violet dries out because of heat in 30 seconds, it will be at a much higher concentration and that could be what
results in all violet/magenta looking color or black that we see in your photo. You probably need to wait until your
heat from fixing is dissipated, then add the few drops of crystal violet. that is how I would interpret your procedure list.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/c6ca85a1-3c5e-85e1-c04b-1aa0c335a5c3%40industromatic.com.
For more options, visit https://groups.google.com/d/optout.