For the gel box, you can get away with a plastic sandwich box from the dollar store, two pieces of stainless steel wire, and a couple of 9V batteries stacked end-to-end. Works just fine...
-- As for the gel, desert grade agar-agar does work, but it's more opaque, and you won't get very sharp bands because it's more heterogeneous. If you just want to know whether or not you got a single band of approximately the right size, that may be enough though.
I'm a little puzzled about the fact that you said you want a solution for places where you can't expect to find agarose, and yet precast gels are OK? It would seem to me that agarose should be much easier to find than precast gels.
Patrik
On Thursday, October 12, 2017 at 1:09:04 PM UTC-7, Abizar Lakdawalla wrote:
On Thursday, October 12, 2017 at 1:09:04 PM UTC-7, Abizar Lakdawalla wrote:
On Thu, Oct 12, 2017 at 10:19 AM, Ra Ramana <emailrav...@gmail.com> wrote:--Objective: Easy & quick ways to id DNA after endpoint PCR in places where one can't expect to find agarose etc. available easily.Pre-cast gels will be good. (No gels & alternative systems such as imaging a la PCR + optics integrated will be ideal any time)Currently doing: Building some open source/ low-cost regular gel boxes & trans-illuminators. Towards this, revisting threads from 2012 such as https://groups.google.com/forum/?hl=en#!msg/diybio/ & making these open design systems [@Cameron from Biocurious, couldn't find your designs/ files. Please share them again including any updates. ]UO6JnUTjE_Y/xXrt_vO5yXoJ; context-place=forum/diybio I've the blue gel system from MiniPCR guys and it's appreciated much around here (in India). But something cheaper & without need for gel casting etc., will be better.The only fast gels systems available that I find are : E-gel (Thermo) and Flashgel (Lonza) both of which are expensive.Seeking:Better/ low-cost designs/suggestions for gel + transilluminator system.Are there open source or low-cost versions available for the quick electrophoresis systems? Pre-cast ones.(i think e-gel patents are almost expiring or are already). Have alternative approaches such as paper-based ones been developedby folks & not yet mainstreamed. (there is a thread here from 2013 on paper electrophoresis with many good references)
What's the take of the community on this?Any recommendations or alternative systems (designs for PCR integrated with imaging at low costs)Thanks,ravi
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