Re: [DIYbio] Making anaerobic media

Use a Gaspack BD to get rid of air, and seal the bag in a thick foil that is not permeable for oxygen. You do not need a different medium just to get the air out of where the culture is. Alternatively you could use a glass bottle with a good seal and make a fluid culture, next remove the air inside the bottle by putting CO2 inside and quickly seal the bottle.

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