Exploring the limits of ultrafast polymerase chain reaction using liquid for thermal heat exchange: A proof of principle
Appl. Phys. Lett. 97, 264101 (2011); https://doi.org/10.1063/1.3530452
http://aip.scitation.org/doi/full/10.1063/1.3530452
"""
We explored the limits of speed by using liquid for thermal exchange rather than metal as in traditional devices, and by testing different polymerases. In a clinical setting, our system equaled or surpassed state-of-the-art devices for accuracy in amplifying DNA/RNA of avian influenza, cytomegalovirus, and human immunodeficiency virus. Using Thermococcus kodakaraensis polymerase and optimizing both electrical and chemical systems, we obtained an accurate, 35 cycle amplification of an 85-base pair fragment of E. coli O157:H7 Shiga toxin gene in as little as 94.1 s, a significant improvement over a typical 1 h PCR amplification.
"""
Appl. Phys. Lett. 97, 264101 (2011); https://doi.org/10.1063/1.3530452
http://aip.scitation.org/doi/full/10.1063/1.3530452
"""
We explored the limits of speed by using liquid for thermal exchange rather than metal as in traditional devices, and by testing different polymerases. In a clinical setting, our system equaled or surpassed state-of-the-art devices for accuracy in amplifying DNA/RNA of avian influenza, cytomegalovirus, and human immunodeficiency virus. Using Thermococcus kodakaraensis polymerase and optimizing both electrical and chemical systems, we obtained an accurate, 35 cycle amplification of an 85-base pair fragment of E. coli O157:H7 Shiga toxin gene in as little as 94.1 s, a significant improvement over a typical 1 h PCR amplification.
"""
On Tue, Feb 16, 2016 at 5:51 PM, Simon Quellen Field <sfield@scitoys.com> wrote:
I ordered some before I posted their price. I'll let you know.I also like the hot air version, especially for the convenient lab appliance I spoke of.Most blow driers have a switch that turns off the heater to blow cold air.Instrumenting that switch might be interesting.The last link I gave was to my AC PID controller project, which could be used to control the temperature of the blow dryer heater.On Tue, Feb 16, 2016 at 5:23 PM, John Griessen <john@industromatic.com> wrote:On 02/16/2016 05:45 PM, William Beeson wrote:
Could this really be built pretty easily?
If those $4.31 pumps can take the hot temp. Why not just find out?
(Me, I still like air as the transfer medium)
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