Re: [DIYbio] Re: Were the DIY CRISPR kits contaminated?

Hey All,

     Quick comment. 

     The contamination that is suggested to have happened occurred over a year ago(1.5 years ago we sent the kit to Bavaria). The EU put out responses and we put out responses also around a year ago, this is like digging up dead bacteria. We don't even work at the same facility we did then, we now ship the bacteria in a different manner. Even so contamination can happen. I have gotten contaminated stabs from other companies and they just send a replacement. This isn't food and as the EU said that if you did somehow manage to eat the bacteria they are not considered deadly and there are common treatments. They also said even if the kit was contaminated kit it was very low risk. I don't know what else I can say in response?

     Wolfgang, we don't even use the same plasmids or bacteria that were shipped to you in 2016. We changed the system completely so it has higher efficiency. Sorry, it didn't work for you. I didn't respond to whatever email you sent because I didn't know what you were looking for in a response? I didn't know how I could help? The old kit and DNA was based exactly off a published paper which we verified the results of. So even if you blame our kit you are also saying the peer reviewed paper is wrong. Maybe it is? Who knows? If you feel slighted by us and want a refund I will give it to you.

     We are working on a new kit that should be out soon that inserts GFP into the genome. We know the antibiotic resistance thing is weird to non-scientists and are trying to change that.

You all tell me what it would take to resolve these things and I will see what I can do. Please let me know how I can help.

Thanks,

     Josiah

 

On Sunday, February 4, 2018 at 3:29:41 PM UTC-8, Wolfgang Nellen wrote:

From the Germans (science bridge): still the same. Got  new bacteria from Don Court, we get StrepR but no correct editing. Our post on Odiham Page is not released. Zayner does not respond to mail.

On 15 Jan 2018 03:15, "August Pamplona" <cosm...@gmail.com> wrote:

On Tuesday, December 26, 2017 at 9:01:47 AM UTC-5, Wolfgang Nellen wrote:

Did you get any response from the Germans yet?
Concerning communication: is there anything like a Facebook site where all communities could talk to each other and discuss issues like the kit? I guess the authorities do not have a clue where the DIY communities are!

Hi,

        There are some. Try https://www.facebook.com/groups/diybio/ and https://www.facebook.com/groups/769326966539324/ . This might be relevant too: https://www.facebook.com/groups/biohackacademy/ 

        Also, if you are into fungi these might be sort of relevant: https://www.facebook.com/groups/FungalSequencing/ & https://www.facebook.com/groups/fungalmaterials/

        There are many other possible matches.

August

 

On 22 Dec 2017 20:00, "Vanessa lorenzo toquero" <vloren...@gmail.com> wrote:

Hi,

I introduce myself. Vanessa Lorenzo from Hackuarium biohackerspace in Renens, Switzerland, co-leading it together with Rachel Aronoff and the board,

Just to inform that we had a kit from the Odin at the lab. We decided to get in contact with the German authorities who suggested that our kit might also be contaminated. We sent them the kit.

We also got better informed on how our community could contribute better to the DIY Bio movement. A more dynamic dialogue between the authorities and the affected peers of the DIY Bio movement would had been more desirable but we are now navigating between different landscapes with different time scales and communication protocols. We should push for a better more transparent ones, and of course our door is literally transparent and always open on Wednesdays or on appointment (basically cause we are volunteers and have side jobs or even lives!). Having a constructive and responsible attitude towards the issues we face could help shaping the science we dream of.

Best regards

Vanessa

El viernes, 22 de diciembre de 2017, 9:52:09 (UTC+1), Jonathan Ferooz escribió:

Nathan I think you are wrong, in Belgium we are very interested to collaborate with other groups (and I'm sure in other EU countries too). But in contrast with some other groups we are a small group with no funding.
What do you think we can do?
We are pushing, at our level, to show what is the DIYbio and hope that we'll can adapt our laws to facilitate the DIYbio.
In europe just have the right to put GFP in a genome it's a fight with the authorities.
If other groups want to collaborate with us in Belgium, we are open.
We are not making the show on TV, Nature or other, but yes, we are there.

Le jeudi 21 décembre 2017 23:20:55 UTC+1, Nathan McCorkle a écrit :

I am surprised this has got any more attention, given the lack of response the EU biohackers have provided here on this mailing list. It gives the impression that EU biohackers are not interested in collaboration, and are making noise for some self-serving goals. This behavior only makes me wary of EU biohackers. Very sad.

--

-Nathan

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