Re: [DIYbio] DIY electroporator

sorry for the late reply.No i am building from scratch with an external power supply.I have enclosed the diagram of circuit .the resistor R2 value I cant figure out how to calculate and its wattage.I took this value randomly from papers I read.The power supply I will be using is glassman power supply which can supply  output voltage=0-40KV and output current =1mA

On Sat, Mar 17, 2018 at 11:31 PM, John Griessen <john@industromatic.com> wrote:

On 03/17/2018 11:57 AM, nisha p wrote:

I am just making a prototype ,so i haven't yet decided which protocol will follow yet.I have biorad 1mm,2mm cuvettes.And sir can you explain to me why in some electroporator circuits capacitor is in series with cuvette and in other, they are in parallel?

If they seem to be parallel or series they may be for different purpose. If in parallel,  they could be shaping the pulse.
If in series, they could be storing energy to be dumped by a big knife switch with huge sparks, or they could be for pulse shaping also.  A big knife switch is good for movies like Frankenstein, but not necessary and generates lots of radio interference,
so not used in real life.

Mine works with output caps some parallel, some series to make a volt doubler.  The transformer turns ratio gets output to 1500V and doubler approaches 3kV, but does not get all the way there because of losses.

What circuits are you reverse engineering?  Old Bio-Rad?


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