Re: [DIYbio] DIY electroporator

On 03/17/2018 11:57 AM, nisha p wrote:
> I am just making a prototype ,so i haven't yet decided which protocol will follow yet.I have biorad 1mm,2mm cuvettes.And sir can
> you explain to me why in some electroporator circuits capacitor is in series with cuvette and in other, they are in parallel?


If they seem to be parallel or series they may be for different purpose. If in parallel, they could be shaping the pulse.
If in series, they could be storing energy to be dumped by a big knife switch with huge sparks, or they could be for pulse shaping
also. A big knife switch is good for movies like Frankenstein, but not necessary and generates lots of radio interference,
so not used in real life.

Mine works with output caps some parallel, some series to make a volt doubler. The transformer turns ratio gets output to 1500V
and doubler approaches 3kV, but does not get all the way there because of losses.

What circuits are you reverse engineering? Old Bio-Rad?


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