Fwd: Fwd: [DIYbio] DIY lab equipment

Date: Sun, 22 Apr 2018 09:44:32 -0400
From: Tom Knight

My email isn't liked by the group, perhaps you could forward this.

Also, I forgot the centrifuge.
(10) Centrifuge. Eppendorf 5424 or 5418 are my current favorites. Both in current production, but available used. Consider a 5415,
but be aware that parts are no longer available.
$800-1000

> A minimal bench setup:
> (1) pipettors. Choose a brand and style and stick with it. You want to only have one type of tip in stock. Good brands are
> Eppendorf, Gilson, Rainin. Know the difference between LTS tips and other types. You need at least 1000ul, 200 ul, 20 ul. 2 ul
> is useful.
> $100 each
>
> (2) vortexer. Get an old, rusty, VWR Vortex Genie. They work better than most of the new ones.
> $100
>
> (3) little picofuge. Get a VWR Galaxy C1413 (really made by Tomy) with the 6-place tube holder.
> $100
>
> (4) Bunsen burner. Many choices, but the pro version is the Fireboy. Watch out, the CV360 butane tanks used by many of these are
> no longer made or available at reasonable prices. You want to use the Coleman propane camp tanks.
> $200-400
>
> (5) PCR machine. I'd get an old Biorad (really MJ) PTC-200 with a 96 well alpha block. Many other choices, this is mine.
> $800-2000
>
> (6) incubator Recycle an old hybridization oven. The Illumina ones often fail and can be fixed with a simple fan swap. Boekel
>  ones are inexpensive and work well, even with digital controls
> $100-300
>
> (7) Serological pipettor. I'd recommend a used Drummond (electric only!). May need to replace the battery. Easiest in the newer
> small sized ones with no bottom bulge.
> $100
>
> (8) Autoclave. Yamato SM-310 is great for a small lab.
> $1000-2000
>
> (9) Water bath. Use a kitchen Sous-Vide unit that has a pump and accurate temperature control. Amazon.
>
>
>>
>> On 04/21/2018 10:58 PM, Lauren R wrote:
>>> Good points!  That's true--you can build a lab that's not bad over time.  I'll keep an eye out and watch equip on eBay.


--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/648f469c-b4f1-924c-ad05-22d1902f8d3b%40industromatic.com.
For more options, visit https://groups.google.com/d/optout.